CELA3B antibody

SKU: orb2276348

Description

CELA3B antibody validated for immunohistochemistry on 76 different Normal Tissues

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Pancreas: Acinar cells should show a strong CELA3B immunostaining with apical predominance. Negative Control: Kidney: CELA3B staining should be absent in all epithelial cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: MSVA-410M
• Immunogen: Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Chymotrypsin like elastase family member 3B (CELA3B); ELA3B; Elastase IIIB; Protease E

Acinar cell carcinoma of the pancreas exhibiting a strong CELA3B immunostaining of all tumor cells.

CELA3B immunostaining is absent in kidney tissue.

Normal pancreas with strong CELA3B positivity of acinar cells.

Quick Database Links

UniProt

P08861