Cdk6 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb559223
• Category: Antibodies
• Description: Cdk6 Recombinant Rabbit Monoclonal Antibody
• Target: CDK6
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant human Cdk6 protein, full length
• UniProt ID: Q00534
• MW: 37 kDa
• Tested applications: FC, ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: 4F7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CDK 6; Cell division protein kinase 6; Crk 2; Crk2
• Research Area: Epigenetics and Nuclear Signaling
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary Ab, dilution: 1:2000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: Jurkat, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 37 kDa, Observed MW: 37 kDa.

Cell line: HeLa, Fixation: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary Ab, dilution: 1:50, Secondary Ab: Goat Anti-Rabbit IgG, Unlabelled control: The cell without incubation, with primary antibody and secondary antibody, (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb559223.

Cell line: HeLa, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab, dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb559223.