CD79a Antibody

• Catalog Number: orb534000
• Category: Antibodies
• Description: The CD79a protein together with the related CD79b protein, forms a dimer associated with membrane-bound immunoglobulin in B-cells, thus forming the B-cell antigen receptor (BCR). CD79a plays multiple and diverse roles in B cell development and function. The CD79a/b heterodimer associates non-covalently with the immunoglobulin heavy chain through its transmembrane region, thus forming the BCR along with the immunoglobulin light chain and the pre-BCR when associated with the surrogate light chain in developing B cells. Association of the CD79a/b heterodimer with the immunoglobulin heavy chain is required for surface expression of the BCR and BCR induced calcium flux and protein tyrosine phosphorylation. [Wiki]
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Amino acids 202-216 (GTYQDVGSLNIADVQ) were used as the immunogen for the recombinant CD79a antibody.
• UniProt ID: P11912
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
• Application notes: The stated application concentrations are suggested starting points. Titration of the CD79a antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: CDLA79a-3R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry testing of human Raji cells with recombinant CD79a antibody (clone CDLA79a-3R); Red = isotype control, Blue = CD79a antibody.

Western blot testing of human Raji cell lysate with recombinant CD79a antibody (clone CDLA79a-3R). Expected molecular weight: 25-47 kDa depending on glycosylation level.

Immunofluorescent staining of PFA-fixed human Raji cells with recombinant CD79a antibody (green, clone CDLA79a-3R) and Reddot nuclear stain (red).

IHC testing of FFPE human tonsil with recombinant CD79a antibody (clone CDLA79a-3R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA followed by cooling at RT for 20 min.