CD3E Antibody

• Catalog Number: orb1333884
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD3E
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: OTI3E10
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: Full length human recombinant protein of human CD3E(NP_000724) produced in HEK293T cell.
• Antibody Type: Primary Antibody
• Dilution range: WB 1:200~4000, IHC 1:150, IF 1:100
• Conjugation: Unconjugated
• MW: 20.7 kDa
• Target: CD3E
• UniProt ID: P07766
• NCBI: NM_000733
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
• Expiration Date: 12 months from date of receipt.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD3E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD3E. Positive ly

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD3E monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-CD3E monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (1:2000) (1:4000)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (1:2000) (1:4000)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (1:2000) (1:4000)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (1:2000) (1:4000)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD3E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Anti-CD3E mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD3E.