Recombinant FCER2 Antibody / CD23

SKU: orb2636320

Description

CD23 (FCER2) is a type II integral membrane glycoprotein that is expressed on mature B cells, monocytes, eosinophils, platelets and dendritic cells. FCER2 is a low affinity IgE receptor that mediates IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils. FCER2 associates as an oligomer where cooperative binding of at least two lectin domains is required for high affinity IgE binding to FCER2. It may play a role in antigen presentation by B cells by interacting with CD40. FCER2 has been shown to be associated with the Fyn tyrosine kinase. The truncated molecule can be secreted, then function as a potent mitogenic growth factor. FCER2 is expressed on a subpopulation of peripheral blood cells, B-lymphocytes and on EBV transformed B lymphoblastoid cell lines. FCER2 is also detected in neoplastic cells from cases of B cell chronic lymphocytic leukemia and some cases on centroblastic/centrocytic lymphoma.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the FCER2 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: FCER2/6474R
• Immunogen: A recombinant fragment (within amino acids 48-321) of human FCER2/CD23 protein was used as the immunogen for the FCER2 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of purified, BSA-free FCER2 antibody (clone FCER2/6474R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P06734