Caspase-6 rabbit pAb Antibody

SKU: orb764730

Description

Rabbit polyclonal antibody to Caspase-6

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Caspase 6. AA range:223-272
• Molecular Weight: 35
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-CASP6 antibody, anti-MCH2 antibody, anti-Caspase-6 antibody, anti-CASP-6 antibody, anti-Apoptotic protease Mch-2 antibody

Western Blot analysis of various cells using Caspase-6 Polyclonal Antibody diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive control (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunofluorescence analysis of HUVEC cells, using Caspase 6 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Caspase 6 Antibody. The picture on the right is blocked with the synthesized peptide.