CASP8 Antibody

• Catalog Number: orb625440
• Category: Antibodies
• Description: Mouse monoclonal antibody to Caspase 8
• Target: CASP8
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Protein A+G purified
• Immunogen: caspase 8, apoptosis-related cysteine peptidase
• UniProt ID: Q14790
• MW: 50-55 kDa, 25-32 kDa
• Tested applications: ELISA, IF, IHC, IP, WB
• Dilution range: WB: 1:500-1:5000; IP: 1:200-1:2000; IHC: 1:100-1:400; IF: 1:50-1:500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Apoptotic cysteine protease, Apoptotic protease Mc
• Research Area: Apoptotic, Cancer, Epigenetics, Infectious Disease
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using orb625440 (Caspase 8 Antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

IP Result of anti-Caspase 8 (IP: orb625440, 5 ug; Detection: orb625440 1:500) with HepG2 cells lysate 2400 ug.

HepG2 cells were subjected to SDS PAGE followed by western blot with orb625440 (CASP8 antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using orb625440 (Caspase 8 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9).