CALR Antibody (Center)

SKU: orb1925305

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,κ
• Clone No.: 2143CT58.2.1
• Immunogen: This CALR antibody is generated from a mouse immunized with a recombinant protein from human CALR.
• Target: CALR (HGNC:1455)
• Molecular Weight: 48142 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Calreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, grp60, CALR (HGNC:1455), CRTC

Anti-CALR Antibody (Center) at dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CALR Antibody (Center) at dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: SH-SY5Y whole cell lysate. Lane 6: K562 whole cell lysate. Lane 7: NIH/3T3 whole cell lysate. Lane 8: C6 whole cell lysate. Lane 9: CHO whole cell lysat. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-CALR Antibody (Center) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lane 3: HeLa whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: SH-SY5Y whole cell lysate. Lane 6: K562 whole cell lysate. Lane 7: NIH/3T3 whole cell lysate Lane 8: C6 whole cell lysate Lane 9: CHO whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 55 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

CALR (HGNC:1455)

UniProt

P27797