Calponin Antibody

SKU: orb749471

Description

Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
• Reactivity: Human, Rat
• Application Notes:
  Optimal dilution of the anti-Calponin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: CNN1/832
• Immunogen: Recombinant human protein was used as the immunogen for the anti-Calponin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with anti-Calponin antibody (clone CNN1/832).

IHC: Formalin-fixed, paraffin-embedded human uterus stained with anti-Calponin antibody (clone CNN1/832).

IHC: Formalin-fixed, paraffin-embedded rat uterus stained with anti-Calponin antibody (clone CNN1/832).

Immunofluorescent staining of PFA-fixed human K562 cells with anti-Calponin antibody (green, clone CNN1/832) and Reddot nuclear stain (red).

SDS-PAGE analysis of purified, BSA-free Calponin antibody (clone CNN1/832) as confirmation of integrity and purity.

Quick Database Links

UniProt

P51911