CA9 Antibody / Carbonic Anhydrase IX

SKU: orb2635849

Description

CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, CAIX is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant Carbonic Anhydrase IX antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: CA9/9621R
• Immunogen: The extracellular N-terminal proteoglycan-like region was used as the immunogen for the recombinant Carbonic Anhydrase IX antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human HeLa cell lysate with recombinant Carbonic Anhydrase IX antibody. Predicted molecular weight: 50-55 kDa but may be observed at higher molecular weights due to glycosylation.

Western blot testing of human stomach tissue lysate with recombinant Carbonic Anhydrase IX antibody. Predicted molecular weight: 50-55 kDa but may be observed at higher molecular weights due to glycosylation.

IHC staining of FFPE human renal cell carcinoma tissue with Carbonic Anhydrase IX antibody (clone CA9/9621R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Carbonic Anhydrase IX antibody (clone CA9/9621R) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q16790