Recombinant Human Neuropilin-1 Protein (Biotin)

SKU: orb2993772

Description

Biotinylated Recombinant Human Neuropilin-1 is produced by our Mammalian expression system and the target gene encoding Phe22-Lys644 is expressed with a Avi, 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Anti-Human NRP1 mAb(Cat#Norb3002196) at 2μg/ml (100 μl/well) can bind Human Neuropilin-1-Avi-His(Cat#orb2993772). The ED50 of Human Neuropilin-1-Avi-His(Cat#orb2993772) is 9.33 ng/ml.
• Tag: C-Avi-6His
• Molecular Weight: Predicted: 72.5 KDa. Observed: 85-100 KDa, reducing conditions
• Expression Region: Phe22-Lys644
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Biotin

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD304; NRP1; NRPNP1; VEGF165R; BDCA4

Quick Database Links

UniProt

AAH07533.1