Beclin 1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2563484
• Category: Antibodies
• Description: Beclin 1 Recombinant Rabbit Monoclonal Antibody
• Target: BECN1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant human Beclin 1 protein (141-450/450aa)
• UniProt ID: Q14457
• MW: 50 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: IHC-P=1:50-200, WB=1:200-1000, IF=1:50-200, IHC-F=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: 9A19
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Beclin-1; ATG6; beclin1; VPS30; Beclin 1 like prot
• Research Area: Autophagy, Cancer Research, Cell Cycle, Host Immun
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

20 ng rhBeclin 1-His protein per lane probed with Beclin 1 polyclonal antibody respectively, unconjugated (orb2563484) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.

25 ug total protein per lane of various lysates probed with Beclin 1 monoclonal antibody, unconjugated (orb2563484) at 1:200 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Fundus, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Kdiney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Beclin 1 Monoclonal Antibody, Unconjugated (orb2563484) at 1:50 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.