Azide-PEG4-aminoallyl-dUTP

SKU: orb1734019

Description

Azide-PEG 4-aminoallyl-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing Azide-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-catalyzed or Cu(I)-free click chemistry that offers the choice: to introduce a Biotin group (via Alkynes of Biotin or DBCO-functionalized Biotin, respectively) for subsequent purification tasks; to introduce fluorescent group (via Alkynes of fluorescent dyes or DBCO-functionalized fluorescent dyes, respectively) for sub-sequent microscopic imaging; to crosslink the DNA to Azide-or DBCO-functionalized biomolecules e.g.proteins.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT). Spectroscopic Propertie: λmax 289 nm, ε 7.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 796.51 g/mol (free acid); Detected MW: 796.15 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₂₃H₃₉N₆O₁₉P₃
• SMILES: [C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCOCCOCCOCCOCCN=[N+]=[N-])O

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only