Vimentin Antibody

• Catalog Number: orb323201
• Category: Antibodies
• Description: The Vimentin Antibody is suitable for IF, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant protein corresponding to human Vimentin. Purification: Affinity chromatography
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: Affinity chromatography
• Immunogen: Recombinant protein corresponding to human Vimentin.
• UniProt ID: P08670, P31000, P20152
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1:1000-1:3000, IHC-P: 1:200-1:500, IF/ICC: 1:100-1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Vimentin
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 81818, 22352, 7431

Western blot analysis of Vimentin expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 50-57 kD)

Immunohistochemical analysis of Vimentin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Vimentin staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).