Anti-UBE2C [SAIC-41A-1]

• Catalog Number: orb1089976
• Category: Antibodies
• Description: Rabbit monoclonal antibody to UBE2C
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: SAIC-41A-1
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Peptide "GISAFPESDNLFK", derived from the Ubiquitin-conjugating enzyme E2C, conjugated to KLH.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: UBE2C
• UniProt ID: O00762
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Ubiquitin-conjugating enzyme E2 C; UBCH10
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the anti-UBE2C antibody SAIC-41A-1. Paraformaldehyde fixed MCF7 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-41A-1 (orb1089976, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Caco-2 cells with anti-UBE2C SAIC-41A-1. Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-41A-1 (orb1089976) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing NUCLEAR staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089976, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-UBE2C antibody SAIC-41A-1. Caco-2(A) (0.3 µg/ml), MCF7(B) (1 ug/ml) and HeLa(C) (1 ug/ml) cell lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-41A-1 (orb1089976) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.