TM4SF1 Biosimilar Antibody

• Catalog Number: orb1743394
• Category: Antibodies
• Description: Anti-TM4SF1 (AGX101 biosimilar) mAb
• Target: TM4SF1
• Clonality: Monoclonal
• Species/Host: Human Humanized
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P30408
• Tested applications: FC
• Dilution range: Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Biosimilar Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: M3S1TAAL6
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis with 1 μg/mL Anti-TM4SF1 (AGX101 biosimilar) mAb (orb1743394) on Expi293 cells transfected with Human TM4SF1 protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

ELISA plates were pre-coated with 0.5 μg/per well purified human TM4SF1 full length membrane nanoparticles (Cat# orb1743250). Serial diluted Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# orb1743394) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-TM4SF1 (AGX101 biosimilar) mAb binding with TM4SF1 MNPs (Cat# orb1743250) is 4.174 ng/ml.

ELISA plates were pre-coated with Flag Tag TM4SF1-Nanodisc (Cat# orb1743247, 0.2 μg/per well). Serial diluted Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# orb1743394) were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# orb1743394) binding with TM4SF1-Nanodisc (Cat# orb1743247) is 15.97 ng/ml.

FACS analysis of TM4SF1 MNPs (Cat# orb1743250).A. TM4SF1 MNP (Cat# orb1743250) were stained only with Goat anti-human 2nd antibody.B. Negative control MNP samples were stained with Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# orb1743394) at 2 μg/mL, followed by Goat anti-human IgG 488 secondary antibody.C. TM4SF1 MNP (Cat# orb1743250) samples were stained with anti-CCR8 antibody (an irrelevant antibody) at 2 μg/mL, followed by Goat anti-human 2nd antibody.D. TM4SF1 MNP (Cat# orb1743250) samples were stained with Anti-TM4SF1 (AGX101 biosimilar) mAb (Cat# orb1743394) at 2 μg/mL, followed by Goat anti-human 2nd antibody.