Tachykinin Receptor 1 Rabbit Polyclonal Antibody

SKU: orb304618

Description

The Tachykinin Receptor 1 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Tachykinin Receptor 1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, GPCR, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-1000, IF/ICC: 1:100-500
• Reactivity: Canine, Gallus, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Tachykinin Receptor 1. The exact sequence is proprietary.
• Target: TACR1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NK1R; TAC1R; Substance-P receptor; SPR; NK-1 receptor; NK-1R; Tachykinin receptor 1

Western blot analysis of Tachykinin Receptor 1 expression in HEK293T (A), A2780 (B), mouse spleen (C), mouse kidney (D), rat spleen (E), rat kidney (F) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46; 53 kD)

Immunofluorescent analysis of Tachykinin Receptor 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

TACR1

UniProt

P14600, P30548, P25103

NCBI Gene ID

24807, 21336, 6869