Phospho-GSK3 beta/GSK3 Rabbit Monoclonal Antibody

SKU: orb548617

Description

Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: WB 1:1000-5000 IHC 1:50-200 ICC/IF 1:50-200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: IFC-7
• Immunogen: A synthesized peptide derived from human Phospho-GSK3 beta (Ser9)
• Target: Glycogen synthase kinase-3 beta
• Molecular Weight: 185 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glycogen synthase kinase-3 beta; GSK-3 beta; 2.7.11.26; Serine/threonine-protein kinase GSK3B; 2.7.11.1; GSK3B

Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-GSK3 beta (Ser9) Antibody (orb548617) GSK3B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSK3B Antibody (orb548617) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Western blot analysis of GSK3 beta (phospho S9) expression in 293T cell lysates, treated with Calyculin A (orb548617). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3B monoclonal antibody (Catalog # orb548617) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for GSK3B.

IF analysis of immunocytochemical section of HeLa cells treated with Calyculin A using anti-Phospho-GSK3 beta (Ser9) antibody (orb548617) Phospho-GSK3 beta (Ser9) was detected in immunocytochemical section. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-Phospho-GSK3 beta (Ser9) Antibody (orb548617) overnight at 4 &deg.

Quick Database Links

Gene Symbol

Glycogen synthase kinase-3 beta

UniProt

P49841