PAX6 Mouse Monoclonal Antibody

SKU: orb1473934

Description

The PAX6 Antibody is suitable for IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human PAX6. The exact sequence is proprietary.

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB (1/500 - 1/4000), IF/IC (1/10 - 1/50)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgM kappa
• Immunogen: Recombinant fusion protein of human PAX6. The exact sequence is proprietary.
• Target: PAX6
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: crude ascites with 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AN2; Paired box protein Pax-6; Aniridia type II protein; Oculorhombin

Western blot analysis of PAX6 expression in 293 (A) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 50 kD)

Immunofluorescent analysis of PAX6 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

PAX6

UniProt

P26367

NCBI Gene ID

5080