NF-kappaB p65 (Phospho-S276) Rabbit Polyclonal Antibody

SKU: orb214503

Description

The NF-kappaB p65 (Phospho-S276) Antibody is suitable for IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S276 of human NF-kappaB p65 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Infectious Diseases, NF-kB, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
• Reactivity: Bovine, Human, Monkey, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S276 of human NF-kappaB p65 protein. The exact sequence is proprietary.
• Target: RELA
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Western blot analysis of NF-kappaB p65 (Phospho-S276) expression in Hela (A), MCF7 (B), Raw264.7 (C) whole cell lysates. (Predicted band size: 60 kD; Observed band size: 65 kD)

Immunohistochemical analysis of NF-kappaB p65 (Phospho-S276) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Direct ELISA antibody dose-response curve using Anti-NF-kappaB p65 (Phospho-S276) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Quick Database Links

Gene Symbol

RELA

UniProt

Q04207, Q04206

NCBI Gene ID

5970, 19697