Myeloperoxidase/MPO Rabbit Polyclonal Antibody (APC)

SKU: orb2580005

Description

Myeloperoxidase/MPO Rabbit Polyclonal Antibody (APC)

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Metabolism Research

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow Cytometry, Optimal dilutions should be determined by end users.
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MPO, different from the related mouse and rat sequences by one amino acid.
• Target: Myeloperoxidase
• Molecular Weight: 83869 Da
• Purification: Immunogen affinity purified.
• Conjugation: APC

Storage & Handling

• Storage: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
• Form/Appearance: Liquid
• Buffer/Preservatives: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Myeloperoxidase; MPO; 1.11.2.2; Myeloperoxidase; 89 kDa myeloperoxidase; 84 kDa myeloperoxidase; Myeloperoxidase light chain; Myeloperoxidase heavy chain; MPO

Western blot analysis of MPO using anti-MPO antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions

IHC analysis of MPO using anti-MPO antibody. MPO was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MPO Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen

MPO was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-MPO Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used

Flow Cytometry analysis of HL-60 cells using anti-MPO antibody. Overlay histogram showing HL-60 cells stained with orb2580005 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPO Antibody for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control

Quick Database Links

Gene Symbol

Myeloperoxidase

UniProt

P05164