CD81 Antibody

SKU: orb43972

Description

Mouse Monoclonal to CD81.

Research Area

Epigenetics, Immunology, Infectious Diseases

Images & Validation

• Tested Applications: FC, ICC, IHC-P, IP, WB
• Reactivity: Feline, Human, Rabbit
• Application Notes:
  Flow cytometry: recommended dilution: 1 μg/ml.Western blotting: recommended dilution: 1-2 μg/ml; positive control: Jurkat cells, non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: M38
• Immunogen: MOLT-4 (human T-ALL cell line)
• Target: CD81
• Purification: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

S5.7, CVID6, TAPA1, TSPAN28

Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD81 (M38) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD81 (M38) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Western blotting analysis of human CD81 using mouse monoclonal antibody M38 on lysate of Jurkat cell line under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-human CD81 monoclonal antibody M38 followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD81 at approximately 25 kDa.

Quick Database Links

Gene Symbol

CD81

UniProt

P60033

NCBI Gene ID

975