CD261 Antibody

• Catalog Number: orb154420
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD261 which is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to activated CD261 which is expressed in most human tissues including spleen, peripheral blood leucocytes, thymus and in a variety oFT tumour-derived cell lines. It plays a role in inducing cell death. The binding oFT TRAIL to DR4 triggers the activation oFT pro-caspases 8 and 10, leading to apoptosis.
• Target: CD261
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.
• UniProt ID: O00220
• Tested applications: FA, FC, ICC, IP
• Application notes: Functional application: Soluble antibody DR-4-02 blocks apoptosis triggered by a ligand (TRAIL). Plastic-immobilized (cross-linked) DR-4-02 antibody induces apoptosis in sensitive cells. Recommended dilution of antibody: 2-3 μg/ml in cultivation medium, final concentration of TRAIL: 20-200 ng/ml; it is recommended to add the antibody 15 min before addition of TRAIL. Flow cytometry: Recommended dilution: 2-4 μg/ml.
• Antibody Type: Primary Antibody
• Clone Number: DR-4-02
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DR4, APO2, TNFRSF10A, TRAILR1, TRAIL-R1
• Research Area: Apoptotic, Cancer, Epigenetics, Neuroscience, Sign
• Note: For research use only
• Entrez: 8797
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis of TRAIL-R1 expression on the surface of hematopoietic cell lines. Cells were stained with purified anti-TRAIL-R1 purified antibodies (low endotoxin) followed by Goat anti-mouse IgG PE.