CD1a Antibody (APC)

• Catalog Number: orb43817
• Category: Antibodies
• Description: Mouse Monoclonal to CD1a.
• Target: CD1a
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: APC
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: Human thymocytes
• UniProt ID: P06126
• RRID: AB_10993726
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Antibody Type: Primary Antibody
• Clone Number: HI149
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: T6, Leu-6, HTA1, FCB6
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Entrez: 909
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1a (HI149) APC antibody (concentration in sample 0.5 µg/ml) and anti-human CD11c (BU15) PE antibody (20 µl reagent per milion cells in 100 µl of cell suspension).

Separation of CD11c positive cells stained using CD1a (HI149) APC antibody (concentration in sample 0.5 µg/ml, red-filled) from CD11c positive cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 0.5 µg/ml, same as CD1a APC concentration, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells.

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD1a (HI149) APC antibody (concentration in sample 0.5 µg/ml).