HLA Class I Antibody (FITC)

SKU: orb44578

Description

Mouse monoclonal antibody against HLA-A conjugated to FITC

Images & Validation

• Tested Applications: FC
• Reactivity: Bovine, Feline, Human, Primate
• Application Notes:
  Flow cytometry: Recommended dilution: 1-3 μg/ml.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Clone No.: W6/32
• Immunogen: Membrane of human tonsil cells
• Target: HLA Class I
• Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: FITC

Storage & Handling

• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Separation of human leukocytes (red-filled) from HLA Class I negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA Class I (W6/32) FITC antibody (concentration in sample 3 μg/ml).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human HLA Class I (W6/32) FITC antibody (concentration in sample 3 μg/ml).

Quick Database Links

Gene Symbol

HLA Class I

NCBI Gene ID

35