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His Tag Antibody
Description
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | WB 1:1000 |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | DM1 |
| Target | His Tag |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of his-tag fusion protein using His-Tag antibody (orb1291195) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H L) at 1:5000 dilution.His-tag fusion protein: 70kDa, 20ng per lane.Blocking buffer: 5% nonfat dry milk in TBST.Exposure time: 1s.
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His Tag Antibody (orb1291195)
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