GAP43 (Phospho-S41) Rabbit Polyclonal Antibody

SKU: orb382520

Description

The GAP43 (Phospho-S41) Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S41 of human GAP43 protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500:2000, IF/ICC: 1:50:100
• Reactivity: Bovine, Gallus, Human, Monkey, Mouse, Rat, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S41 of human GAP43 protein. The exact sequence is proprietary.
• Target: GAP43
• Purity: The antibody was purified by immunogen affinity chromatography
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46

Western blot analysis of GAP43 (Phospho-S41) expression in HeLa PMA-treated (A), SHSY5Y (B) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 24 kD)

Immunofluorescent analysis of GAP43 (Phospho-S41) staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

GAP43

UniProt

P17677, P06837, P07936

NCBI Gene ID

14432, 29423, 2596