Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)]

• Catalog Number: orb357996
• Category: Antibodies
• Description: Recombinant monoclonal antibody to Flavivirus group.
• Target: Flavivirus group antigen
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG
• Conjugation: Unconjugated
• Reactivity: Virus
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: Dengue Virus type 2 antigens.
• Tested applications: ELISA, FC, IF, IHC-P, WB
• Clone Number: D1-4G2-4-15 (4G2)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Protein E; Envelope Protein
• Research Area: Infectious Disease & Virology
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection of Zika virus by immunofluorescence. Immunofluorescence images of Vero cells infected with ZIKV after 30h infection as well as controls. Cells were fixed in 4% PFA for 30 min at RT. Primary antibody (orb357996, rabbit IgG) staining was performed in 0.3% Triton X-100 at 1:20 dilution overnight at 4°C, detected using a Goat Anti-Rabbit IgG (AF568, 1:500) for 1h at RT and counterstained with DAPI.

ELISA on Denge Virus-Like Particles using orb357995 Dengue Virus Serotype 2 VLPs were coated onto a plate at 5 µg/ml. Anti-flavivirus antibody (orb357995) added to plate in a 3-fold serial dilution starting at 1000 ng/ml. Detection performed using HRP labelled goat anti-mouse IgG.