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DDDDK tag Antibody
Description
Images & Validation
−| Tested Applications | ICC, WB |
|---|---|
| Reactivity | All |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | F-tag-01 |
| Immunogen | Synthetic peptide: DYKDDDDK conjugated to KLH |
| Target | DDDDK tag |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunocytochemistry (confocal microscopy) of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594); B - cell nuclei stained with DAPI (blue); C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue).
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DDDDK tag Antibody (orb43789)
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