Cytokeratin 19 Mouse Monoclonal Antibody

SKU: orb1474032

Description

The Cytokeratin 19 Antibody is suitable for IF, IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human Cytokeratin 19. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB (1/1000 - 1/4000), IH (1/50 - 1/200)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Immunogen: Recombinant fusion protein of human Cytokeratin 19. The exact sequence is proprietary.
• Target: KRT19
• Purification: This antibody is purified through a protein G column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Keratin, type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19

Western blot analysis of Cytokeratin 19 expression in HepG2 (A), MCF7 (B) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 44 kD)

Immunohistochemical analysis of Cytokeratin 19 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Cytokeratin 19 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

KRT19

UniProt

P08727

NCBI Gene ID

3880