Cytokeratin 18 Antibody

• Catalog Number: orb43746
• Category: Antibodies
• Description: Mouse monoclonal antibody to Cytokeratin 18
• Target: Cytokeratin 18
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Immunogen: Human breast carcinoma cell line PMC-42.
• UniProt ID: P05783
• RRID: AB_10989892
• Tested applications: ELISA, ICC, IHC-P, IP, WB
• Application notes: Western blotting: Recommended dilution: 1-2 μg/ml.Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: colon, skin. Immunocytochemistry: Positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line.ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line; the antibody DA-7 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. orb43702).
• Antibody Type: Primary Antibody
• Clone Number: DA-7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: K18, CK18, CYK18, KRT18
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 3875

Immunocytochemistry staining of cytokeratin 18 in Hep-2 cells using mouse monoclonal antibody DA-7 (orb43746, diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green), cell nuclei stained with PI (1 µg/ml; orange).

Immunohistochemistry staining of human colon (paraffin sections) using anti-cytokeratin 18 (clone DA-7).

Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DA-7 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for cytokeratin 18 at approximately 46 kDa.