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Anti-cytochrome c [3.7C4]

SKU: orb758902

Description

Mouse monoclonal antibody to Cytochrome C

Research Area

Cell Biology

Images & Validation

Tested ApplicationsELISA
ReactivityMouse

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.3.7C4
ImmunogenThis antibody was raised by immunising OVA-primed mice with cytochrome c-OVA.
Targetcytochrome c
PurityPurified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Cytochrome c oxidase subunit 1; CYT; cytc; Cytochrome c oxidase polypeptide I

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Anti-cytochrome c [3.7C4]

Immunofluorescence staining of fixed NIH3T3 cells with anti-cytochrome c antibody 3.7C4. Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 3.7C4 (orb758903) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758903, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Anti-cytochrome c [3.7C4] (orb758902)

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0.2 mg
$ 620.00
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