Anti-CD44 (Indian blood group) [SAIC-01B-4D8]

SKU: orb1090001

Description

Rabbit monoclonal antibody to CD44 (Indian blood group)

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: SAIC-01B-4D8
• Immunogen: Peptide "TFIPVTSAK" derived from CD44, a hyaluronic acid receptor mediating cell-cell and cell-matrix interactions , conjugated to KLH.
• Target: CD44 (Indian blood group)
• Purity: Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

LHR; MDU2; MDU3; MIC4; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor

Flow cytometry using the Anti-CD44 (Indian blood group) antibody SAIC-01B-4D8. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SAIC-01B-4D8 (orb1090001, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-CD44 (Indian blood group) SAIC-01B-4D8. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of SAIC-01B-4D8 (orb1090001) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1090001, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD44 (Indian blood group)

UniProt

P16070