Anti-CD33 (gemtuzumab biosimilar) mAb

• Catalog Number: orb689320
• Category: Antibodies
• Description: Anti-CD33 (gemtuzumab biosimilar) mAb
• Target: CD33
• Clonality: Monoclonal
• Species/Host: Human Humanized
• Isotype: IgG4
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Powder
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P20138
• Tested applications: FC
• Dilution range: Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Biosimilar Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD33;SIGLEC3;gp67
• Note: For research use only

HEK293 cell line transfected with irrelevant protein (B) and human CD33 (A) were surface stained with anti-CD33 neutralizing antibody 1 μg/ml (gemtuzumab) followed by Alexa 488-conjugated anti-human IgG secondary antibody.

Flow cytometry analysis of antigen binding of anti-human CD33 mAb (orb689320). (A) A clear peak shift of orb689320 was seen compared to the control when incubated with CD33-expressing 8226 cells, indicating strong binding of orb689320 to CD33. Antibodies were incubated at 2 μg/mL.