CCL14 Rabbit Polyclonal Antibody

SKU: orb1147786

Description

Anti-CCL14 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Human.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human ELISA, 0.1-0.5 μg/ml, -
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CCL14 recombinant protein (Position: T20-N93).
• Target: C-C motif chemokine 14
• Molecular Weight: 250 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C C motif chemokine 14; CC 1; CC 3; CCL14; Chemokine CC 1/CC 3; CKb1; HCC 1; HCC 1(1 74); HCC 1/HCC 3; HCC 3; MCIF; NCC 2; NCC2; SCYA14; SCYL2; Small inducible cytokine A14; SY14

IHC analysis of CCL14 using anti-CCL14 antibody. CCL14 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CCL14 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of CCL14 using anti-CCL14 antibody. CCL14 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CCL14 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Quick Database Links

Gene Symbol

C-C motif chemokine 14

UniProt

Q16627