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| Catalog Number | orb1173655 |
|---|---|
| Category | Antibodies |
| Description | Anti-BAFF (belimumab biosimilar) mAb |
| Target | BAFF |
| Clonality | Monoclonal |
| Species/Host | Human |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Powder |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| UniProt ID | Q9Y275 |
| Tested applications | ELISA, FC |
| Dilution range | ELISA 1:5000-10000 Flow Cyt 1:100 |
| Application notes | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Antibody Type | Biosimilar Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | TNFSF13BBAFFBLYSCD257DTLTALL1THANKTNFSF20ZTNF4TALL Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human BAFF, hFc tagged protein (orb689402) can bind Anti-BAFF Neutralizing antibody (orb1173655) in a linear range of 3.2-400 ng/ml. In order to specifically detect orb1173655, mouse anti-human Fab-specific antibody was used as detection antibody.

Flow cytometry analysis with Anti-BAFF mAb 1 μg/ml (belimumab) on Expi293 cells transfected with human BAFF (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
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