Anti-Apg3/ATG3 Antibody

• Catalog Number: orb402477
• Category: Antibodies
• Description: Anti-Apg3/ATG3 Antibody. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Monkey, Mouse
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Apg3 recombinant protein (Position: M1-T244). Human Apg3 shares 96.7% and 96.3% amino acid (aa) sequence identity with mouse and rat Apg3, respectively.
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 36-40 kDa
• UniProt ID: Q9NT62
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Ubiquitin-like-conjugating enzyme ATG3; 6.3.2.-; A
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Apg3 using anti-Apg3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apg3 antigen affinity purified polyclonal antibody at 0.25 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Apg3 at approximately 36-40 kDa. The expected band size for Apg3 is at 36 kDa.