Actin pan Antibody

SKU: orb705413

Description

The Actin pan Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within human Actin pan. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:300
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Actin pan. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% rAlbumin and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

ACTB; Actin cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin cytoplasmic 2; Gamma-actin

Western blot analysis of Actin pan expression in Hela (A), HepG2 (B), MCF7 (C) whole cell lysates. (Predicted band size: 41; 42 kD; Observed band size: 42 kD)

Immunohistochemical analysis of Actin pan staining in human cardiac muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of Actin pan staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemical analysis of Actin pan staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P60709, P63261, Q9BYX7

NCBI Gene ID

71, 60