Aminoallyl-dUTP-XX-ATTO-425

SKU: orb1733871

Description

Aminoallyl-dUTP-XX-ATTO425 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-XX-ATTO425/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-XX-ATTO425/80-70% dTTP (PCR), 30-50% Aminoallyl-dUTP-XX-ATTO425/70-50% dTTP (Nick Translation).

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 439 nm, λem 485 nm, ε 45.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 1132.97 g/mol (free acid); Detected MW: 1132.36 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₄₆H₆₇N₆O₂₁P₃
• SMILES: C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)NC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCN1c2cc3oc(=O)c(C(=O)OCC)cc3cc2C(C)CC1(C)C

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only