Aminoallyl-dUTP-XX-AF488

SKU: orb1733424

Description

Aminoallyl-dUTP-XX-AF488 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. AF488 (also known as Alexa Fluor 488®) is a hy-drophilic dye with excellent photostability compared to fluorescein. Recommended Aminoallyl-dUTP-XX-AF488/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-XX-AF488/70-50% dTTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λexc 494 nm, λem 515 nm, ε 73.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 1265.99 g/mol (free acid); Detected MW: 1265.18 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₄₅H₅₄N₇O₂₆P₃S₂
• SMILES: C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)CCCCCNC(=O)c1ccc(c(C(=O)O)c1)c1c2c(oc3c1ccc(=[NH2+])c3S(=O)(=O)[O-])c(c(cc2)N)S(=O)(=O)O

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only