Aminoallyl-dUTP-Cy5

SKU: orb64137

Description

Aminoallyl-dUTP-Cy5 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-Cy5/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-Cy5/70-50% dTTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 649 nm, λem 670 nm, ε 250.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 1162.01 g/mol (free acid); Detected MW: 1161.23 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₄₅H₅₈N₅O₂₁P₃S₂
• SMILES: C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCN1/C(=C/C=C/C=C/C2=[N+](CC)c3c(C2(C)C)cc(S(=O)(=O)[O-])cc3)/C(c2c1ccc(c2)S(=O)(=O)O)(C)C

Storage & Handling

• Storage: store at -20 °C
• Hazard Information: Non-Toxic
• Disclaimer: For research use only