Aminoallyl-dUTP-Cy3

SKU: orb64138

Description

Aminoallyl-dUTP-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-Cy3/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-Cy3/50% dTTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: Primer Extension with Klenow fragment; PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 550 nm, λem 570 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5), CF 2600.08.

Key Properties

• MW: Theoretical MW: 1135.97 g/mol (free acid); Detected MW: 1135.21 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₄₃H₅₆N₅O₂₁P₃S₂
• SMILES: P(=O)(OP(=O)(O)O)(O)OP(=O)(O)OC[C@H]1O[C@H](C[C@@H]1O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCCCN1c2ccc(S(=O)(=O)O)cc2C(C)(C)/C/1=C\C=C\C1=[N+](c2ccc(cc2C1(C)C)S(=O)(=O)[O-])CC

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only