Aminoallyl-dUTP-ATTO-680

SKU: orb1733883

Description

Aminoallyl-dUTP-ATTO680 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO680/dTTP ratio for PCR and Nick Translation: 30% Aminoallyl-dUTP-680/70% dTTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 681 nm, λem 698 nm, ε 125.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 1030.83 g/mol (free acid); Detected MW: 1030.20 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₃₉H₄₉N₆O₁₉P₃S
• SMILES: [C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OP(=O)(OP(=O)(O)O)O)O)n1c(=O)[nH]c(=O)c(c1)/C=C/CNC(=O)CCCN1c2c(C(=CC1(C)C)CS(=O)(=O)[O-])cc1c(c2)Oc2c(=N1)cc1c(=[N+](CCC1)CC)c2)O

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only