Aminoallyl-dUTP-ATTO-643

• Catalog Number: orb638831
• Category: Tools
• Description: Aminoallyl-dUTP-643 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. ATTO 643 is a hydrophilic dye with excellent photostability compared to fluorescein. Recommended Aminoallyl-dUTP-643/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-643/70-50% dTTP.
• Form/Appearance: filtered solution (30 kDa) in 10 mM Tris-HCl; Color: blue; pH: 7.5 ± 0.5
• Concentration: 1.0 mM-1.1 mM
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 1340.4 g/mol (free acid)
• Application notes: Incorporation into DNA/cDNA by: PCR with Taq polymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. ; Spectroscopic Propertie: λexc 643 nm, λem 665 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Formula: C₁₂H₁₉N₃O₁₄P₃-ATTO 643
• SMILES: C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)N*
• Storage: store at -20 °C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.