Aminoallyl-dUTP-ATTO-425

SKU: orb1733889

Description

Aminoallyl-dUTP-ATTO425 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO425/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-ATTO425/80-70% dTTP (PCR), 30-50% Aminoallyl-dUTP-ATTO425/70-50% dTTP (Nick Translation).

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 439 nm, λem 485 nm, ε 45.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).

Key Properties

• MW: Theoretical MW: 906.66 g/mol (free acid); Detected MW: 906.16 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₃₄H₄₅N₄O₁₉P₃
• SMILES: O=C(OCC)c1cc2c(oc1=O)cc1N(CCCC(=O)NC/C=C/c3cn(c(=O)[nH]c3=O)[C@H]3C[C@@H]([C@H](O3)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)C(C)(C)CC(C)c1c2

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only