AMCA-6-dUTP

• Catalog Number: orb178359
• Category: Tools
• Description: AMCA-6-dUTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended AMCA-6-dUTP/dTTP ratio for PCR and Nick Translation: 20-30% AMCA-6-dUTP/80-70% dTTP (PCR), 30-50% AMCA-6-dUTP/70-50% dTTP (Nick Translation).
• Concentration: 1.0 mM-1.1 mM
• Form/Appearance: filtered solution (30 kDa) in 10 mM Tris-HCl; pH: 7.5 ± 0.5
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 851.58 g/mol (free acid); Detected MW: 851.16 g/mol (free acid)
• SMILES: C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCCCCNC(=O)Cc1c(c2c(oc1=O)cc(cc2)N)C
• Formula: C₃₀H₄₀N₅O₁₈P₃
• Storage: store at -20 °C
• Alternative names: Aminoallyl-dUTP-AMCA, Aminomethylcoumarin-6-dUTP,
• Note: For research use only
• Application notes: Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. < br>< b>Spectroscopic Propertie: λexc 350 nm, λem 450 nm, ε 19.0 L mmol-1 cm-1 (Tris-HCl pH 7.5).
• Expiration Date: 12 months from date of receipt.