Alpha smooth muscle Actin Rabbit Polyclonal Antibody (PE-Cy7)

• Catalog Number: orb917666
• Category: Antibodies
• Description: Alpha smooth muscle Actin Rabbit Polyclonal Antibody (PE-Cy7)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF
• Predicted Reactivity: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Actin alpha (301-375/375aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg /test, IF=1:100-500
• Conjugation: PE/Cy7
• Target: ACTA2
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: alpha sarcomeric Actin; alpha smooth muscle Actin;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ACTA2 antibody (orb917666), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-ACTA2 antibody (orb917666), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.