Alpha smooth muscle Actin Mouse Monoclonal Antibody

• Catalog Number: orb500840
• Category: Antibodies
• Description: Alpha smooth muscle Actin Mouse Monoclonal Antibody
• Target: ACTA2
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Gallus
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein G
• Immunogen: KLH conjugated synthetic peptide derived from human Actin alpha
• UniProt ID: P62736
• MW: 42 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
• Antibody Type: Primary Antibody
• Clone Number: 8B2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: alpha sarcomeric Actin; alpha smooth muscle Actin;
• Research Area: Actina, Arterial, Cell Biology, Cytoskeleton, Deve
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (alpha smooth muscle Actin) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: A431 Cell Lysate at 40 ug Hela Cell Lysate at 40 ug H9C2 Cell Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-alpha smooth muscle Actin (orb500840) at 1/5000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD.

Sample: Lane 1: Large intestine (Mouse) Lysate at 40 ug, Lane 2: Stomach (Mouse) Lysate at 40 ug, Lane 3: Uterus (Mouse) Lysate at 40 ug, Lane 4: Kidney (Mouse) Lysate at 40 ug, Lane 5: Small intestine (Mouse) Lysate at 40 ug, Lane 6: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Lane 7: Stomach (Rat) Lysate at 40 ug, Lane 8: Uterus (Rat) Lysate at 40 ug, Lane 9: A549 (Human) Cell Lysate at 30 ug, Lane 10: Hela (Human) Cell Lysate at 30 ug, Lane 11: A431 (Human) Cell Lysate at 30 ug, Lane 12: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-alpha smooth muscle Actin (orb500840) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 42 kD, Observed band size: 42 kD.