7-Amino-4-methylcoumarin-3-acetic acid

SKU: orb1297479

Description

7-Amino-4-methylcoumarin-3-acetic acid serves as a superior fluorogenic substrate for enteropeptidase compared to GD4K-NA. It is widely used in vitro for the sensitive and continuous assay of this key protease, facilitating research in digestive physiology and recombinant protein processing.

Research Area

Protein Biochemistry

Key Properties

• CAS Number: 106562-32-7
• MW: 233.22
• Purity: 98.38% (May vary between batches)
• Formula: C₁₂H₁₁NO₄
• SMILES: Cc1c(CC(O)=O)c(=O)oc2cc(N)ccc12
• Target: Enteropeptidase (EP),Others
• Solubility: DMSO:55 mg/mL (235.83 mM)

Bioactivity

• In Vitro:
  In this study, we used shift in the emission spectrum of GD(4)K-conjugated 7-Amino-4-methylcoumarin (GD(4)K-AMC) as a fluorogenic method to measure enteropeptidase activity. The kinetic analysis revealed that enteropeptidase has a K(M) of 0.025 mM and a k(cat) of 65 sec(-1) for 7-Amino-4-methylcoumarin, whereas it has a K(M) of 0.5 to 0.6 mM and a k(cat) of 25 sec(-1) for GD(4)K-NA. The optimum pH of 7-Amino-4-methylcoumarin hydrolysis was pH 8.0.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

7 Amino 4 methylcoumarin 3 acetic acid, 7Amino4methylcoumarin3acetic acid, 7-Amino-4-methylcoumarin-3-acetic acid, coumarin dye, fluorophore, flow cytometry, immunofluorescence

Compound Identifiers

PubChem CID

129367