5-TCO-PEG4-dUTP

SKU: orb532289

Description

5-TCO-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting TCO-functionalized DNA/cDNA can subsequently be labeled via Cu(I)-free Click Chemistry (TCO-Tetrazine-Ligation) that offers the choice: to introduce a Biotin group (via Tetrazines of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Tetrazine-functionalized biomolecules e.g. proteins.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: max 240 nm, 10.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 922.70 g/mol (free acid); Detected MW: 922.24 g/mol (free acid)
• Purity: 95% (HPLC)
• Formula: C₃₂H₅₃N₄O₂₁P₃
• SMILES: C(/C=C/c1cn(c(=O)[nH]c1=O)[C@H]1C[C@@H]([C@H](O1)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)NC(=O)CCOCCOCCOCCOCCNC(=O)OC1CCCC=CCC1

Storage & Handling

• Storage: store at -20 C
• Disclaimer: For research use only